Introduction, Platforms, and Challenges of Biofluid microRNA
Introduction to Biofluid microRNA Sequencing Extracellular RNAs (exRNA) have previously been introduced in plasma and other body fluids as novel biomarkers and potential cell-cell communicators. microRNAs (miRNA) have been involved in a wide variety of illnesses, such as heart failure, cancer, and multiple sclerosis, and are the most researched class of exRNAs to date. miRNA…
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Overview of m6A RNA Methylation
What is m6A Modification? Eukaryotic RNA contains over 160 modifications, primarily found on tRNAs and rRNAs. These modifications include m1A, m6A, m7G, m5C, and others. However, due to technological limitations, only a few types of modifications, such as “m6A (N6-methyladenosine)” and m5C, have been extensively studied, with m6A being the most researched. m6A refers to…
Overview of WGBS
Epigenetic modifications, particularly DNA methylation, play an indispensable role in modulating gene expression, cell differentiation, embryogenesis, and disease pathogenesis. WGBS technology has transformed the field of epigenetics by enabling genome-wide, high-resolution mapping of DNA methylation patterns, thereby facilitating in-depth investigations into the molecular mechanisms underlying these biological phenomena. The Principle of WGBS The foundation of WGBS lies…
The Applications Of EM-Seq and Its Promising Potential
EM-seq (Enzymatic Methyl-sequencing), a cutting-edge technique for identifying DNA methylation profiles, is showing great potential in various fields, including epigenetic research, disease diagnosis, and the early detection of cancer. Compared to the traditional whole-genome bisulfite sequencing (WGBS), EM-seq offers several advantages. It allows for the creation of DNA sequencing libraries with superior quality, requires less DNA…